Overview

Teaching: 30 min
Exercises: 30 min

Questions

• How does one reroute flux towards production using gene deletions?

Objectives

• Understand of a growth-coupled design.

Key Points

• cameo.strain_design.OptGene is strain design methods that utilizes genetic algorithms to determine gene (or reaction) knockout combinations that are likely to increase production.

• cameo.strain_design.OptKnock use mixed integer linear programming to compute knockout combinations that couple production to growth.

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