Mass spectrometry-based metabolomics introduction#

The course will introduce MS-based metabolomics data, it’s basic processing and the downstream analysis. The course will be a mix of lectures and hands-on session where we will perform the basic processing steps using metaboigniter, a nextflow workflow, and acore, short for analytical core for the downstream analysis.

Location#

DTU Campus - Building 421

Auditorium A071 from 9.00 to 12.15

  • Introduction with overview of metabolomics and preprocessing

  • Steps in data processing and running metaboigniter (hands-on)

Auditorium A073 from 12.15 to 16.30

  • Lunch

  • Hands-on

    • Data cleaning, normalization and statistical analysis

    • Annotation and Interpretation

Agenda#

Time

Topic

lecturer

09.00 - 09.10

Welcome, coffee, and setup

-

09.10 - 10.30

Introduction with overview of metabolomics and preprocessing

Maria Barranco-Altirriba

10.30 - 10.45

break

-

10.45 - 12.15

Steps in data processing and running metaboigniter (hands-on)

Henry Webel

12.15 - 13.00

Lunch (sandwiches are provided)

-

13.00 - 13.45

Data cleaning, normalization and statistical analysis (lecture)

Felicia Schulz

13.45 - 13.55

break

-

13.55 - 14.40

Data cleaning, normalization and statistical analysis (Hands-On)

Felicia Schulz

14.40 - 14.50

break

-

14.50 - 15.35

Annotation and Interpretation (lecture)

Maria Barranco-Altirriba

15.35 - 15.45

break

-

15.45 - 16.30

Annotation and Interpretation (hands-On)

Henry Webel

Some details to the agenda points#

09.10-10.30 - Introduction with overview of metabolomics and preprocessing#

See the slides for introduction: link

10.45-12.15 - Steps in data processing and running metaboigniter#

Find the instruction here and the slides.

13.00 - 14.40 - Data cleaning, normalization and statistical analysis#

See the slides for the statistical analysis: will be here

14.50 - 16.30 - Data cleaning, normalization and statistical analysis#

Find the notebook here and the slides.