4.10 Summary
| Step | Decision made |
|---|---|
| Design formula | ~ condition (treatment vs control) |
| LFC shrinkage | apeglm method |
| Significance threshold | padj < 0.05 |
| Fold change threshold | |log2FC| ≥ 1 (2-fold) |
| Annotation database | org.EcK12.eg.db (for enrichment, next script) |
Significant genes are saved and ready for gene set enrichment analysis in the next script.
## R version 4.4.1 (2024-06-14)
## Platform: aarch64-apple-darwin20
## Running under: macOS Sonoma 14.3
##
## Matrix products: default
## BLAS: /Library/Frameworks/R.framework/Versions/4.4-arm64/Resources/lib/libRblas.0.dylib
## LAPACK: /Library/Frameworks/R.framework/Versions/4.4-arm64/Resources/lib/libRlapack.dylib; LAPACK version 3.12.0
##
## locale:
## [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
##
## time zone: Europe/Copenhagen
## tzcode source: internal
##
## attached base packages:
## [1] stats4 stats graphics grDevices utils datasets methods
## [8] base
##
## other attached packages:
## [1] plotly_4.12.0 DT_0.34.0
## [3] kableExtra_1.4.0 knitr_1.51
## [5] factoextra_2.0.0 pheatmap_1.0.13
## [7] RColorBrewer_1.1-3 ggpubr_0.6.3
## [9] DESeq2_1.46.0 SummarizedExperiment_1.36.0
## [11] Biobase_2.66.0 MatrixGenerics_1.18.1
## [13] matrixStats_1.5.0 GenomicRanges_1.58.0
## [15] GenomeInfoDb_1.42.3 IRanges_2.40.1
## [17] S4Vectors_0.44.0 BiocGenerics_0.52.0
## [19] reshape2_1.4.5 lubridate_1.9.5
## [21] forcats_1.0.1 stringr_1.6.0
## [23] dplyr_1.2.1 purrr_1.2.2
## [25] readr_2.2.0 tidyr_1.3.2
## [27] tibble_3.3.1 ggplot2_4.0.3
## [29] tidyverse_2.0.0
##
## loaded via a namespace (and not attached):
## [1] rlang_1.2.0 magrittr_2.0.5 otel_0.2.0
## [4] compiler_4.4.1 systemfonts_1.3.2 vctrs_0.7.3
## [7] pkgconfig_2.0.3 crayon_1.5.3 fastmap_1.2.0
## [10] backports_1.5.1 XVector_0.46.0 labeling_0.4.3
## [13] rmarkdown_2.31 tzdb_0.5.0 UCSC.utils_1.2.0
## [16] xfun_0.57 zlibbioc_1.52.0 cachem_1.1.0
## [19] jsonlite_2.0.0 DelayedArray_0.32.0 BiocParallel_1.40.2
## [22] broom_1.0.12 parallel_4.4.1 R6_2.6.1
## [25] bslib_0.10.0 stringi_1.8.7 car_3.1-5
## [28] numDeriv_2016.8-1.1 jquerylib_0.1.4 Rcpp_1.1.1-1.1
## [31] bookdown_0.46 Matrix_1.7-5 timechange_0.4.0
## [34] tidyselect_1.2.1 rstudioapi_0.18.0 abind_1.4-8
## [37] yaml_2.3.12 codetools_0.2-20 lattice_0.22-9
## [40] plyr_1.8.9 withr_3.0.2 S7_0.2.2
## [43] coda_0.19-4.1 evaluate_1.0.5 xml2_1.5.2
## [46] pillar_1.11.1 carData_3.0-6 generics_0.1.4
## [49] emdbook_1.3.14 hms_1.1.4 scales_1.4.0
## [52] glue_1.8.1 lazyeval_0.2.3 tools_4.4.1
## [55] apeglm_1.28.0 data.table_1.18.2.1 locfit_1.5-9.12
## [58] ggsignif_0.6.4 mvtnorm_1.3-6 grid_4.4.1
## [61] bbmle_1.0.25.1 crosstalk_1.2.2 bdsmatrix_1.3-7
## [64] colorspace_2.1-2 GenomeInfoDbData_1.2.13 Formula_1.2-5
## [67] cli_3.6.6 textshaping_1.0.5 S4Arrays_1.6.0
## [70] viridisLite_0.4.3 svglite_2.2.2 gtable_0.3.6
## [73] rstatix_0.7.3 sass_0.4.10 digest_0.6.39
## [76] SparseArray_1.6.2 ggrepel_0.9.8 htmlwidgets_1.6.4
## [79] farver_2.1.2 htmltools_0.5.9 lifecycle_1.0.5
## [82] httr_1.4.8 MASS_7.3-65